Caspase 3 anticorps (Biotin)

N° du produit ABIN2688869

Cet anticorps Lapin Monoclonal détecte spécifiquement Caspase 3 dans ICS. Il présente une réactivité envers Humain et Souris et a été mentionné dans 3+ publications.

Aperçu rapide pour Caspase 3 anticorps (Biotin) (ABIN2688869)

Antigène: Caspase 3 (CASP3)

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp Caspase 3 est conjugé à/à la Biotin

Application: Intracellular Staining (ICS)

Clone: C92

Détail du produit anti-Caspase 3 anticorps (Biotin)

Marque: BD Pharmingen™

Attributs du produit: The caspase family of cysteine proteases plays a key role in apoptosis and inflammation. Caspase-3 is a key protease that is activated during the early stages of apoptosis and, like other members of the caspase family, is synthesized as an inactive pro-enzyme that is processed in cells undergoing apoptosis by self-proteolysis and/or cleavage by another protease. The processed forms of caspases consist of large (17-22 kDa) and small (10-12 kDa) subunits which associate to form an active enzyme. Active caspase-3, a marker for cells undergoing apoptosis, consists of a heterodimer of 17 and 12 kDa subunits which is derived from the 32 kDa pro-enzyme. Active caspase-3 proteolytically cleaves and activates other caspases, as well as relevant targets in the cytoplasm, e.g., D4-GDI and Bcl-2, and in the nucleus (e.g. PARP). This antibody has been reported to specifically recognize the active form of caspase-3 in human and mouse cells. It has not been reported to recognize the pro-enzyme form of caspase-3. Flow cytometric analysis of apoptotic and non-apoptotic populations for active caspase-3. Jurkat cells (Human T-cell leukemia, ATCC TIB-152) were left untreated (left panel) or treated with 4 μM of camptothecin for 4 hr to induce apoptosis (right panel). Cells were washed once in PBS, then fixed and permeabilized using the BD Cytofix/Cytoperm™ Kit (Cat. No. 554714) for 20 min at room temperature (RT), pelleted and washed with BD Perm/Wash™ buffer (component of Cat. No. 554714). Cells were subsequently stained with the biotin rabbit anti- active caspase-3 antibody (clone C92-605). Cells were then washed and resuspended in BD Perm/Wash™ buffer containing FITC-Avidin (5 ng/1x10e6 cells) [Cat. No. 554057] and incubated for 30 min at RT in the dark. Cells were then washed and resuspended in BD Perm/Wash™ buffer before analyzing by flow cytometry. The results show that untreated cells were primarily negative for active caspase-3 (left panel, M1), whereas more than half of the treated cells were positive for active caspase-3 staining (right panel, M2).

BD Pharmingen™ Biotin Rabbit Anti- Active Caspase-3 - Biotin - Clone C92-605 - Isotype Rabbit IgG - Reactivity Hu, Ms - 100 Tests

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Isotype: IgG

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Volume d'échantillon: 20 μL

Restrictions: For Research Use only

Stockage

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Références pour anticorps anti-Caspase 3 (Biotin) (ABIN2688869)

Dai, Krantz: "Interferon gamma induces upregulation and activation of caspases 1, 3, and 8 to produce apoptosis in human erythroid progenitor cells." dans: Blood, Vol. 93, Issue 10, pp. 3309-16, (1999) (PubMed).

Fujita, Tsuruo: "Involvement of Bcl-2 cleavage in the acceleration of VP-16-induced U937 cell apoptosis." dans: Biochemical and biophysical research communications, Vol. 246, Issue 2, pp. 484-8, (1998) (PubMed).

Thornberry, Lazebnik: "Caspases: enemies within." dans: Science (New York, N.Y.), Vol. 281, Issue 5381, pp. 1312-6, (1998) (PubMed).

Détails sur Caspase 3

Antigène: Caspase 3 (CASP3)

Autre désignation: Caspase-3

Sujet: Synonyms: CPP32, Yama, Apopain

Pathways: Apoptose, Caspase Cascade in Apoptosis, Sensory Perception of Sound, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation